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Genomic DNA was removed from RNA samples using AccuRT Genomic DNA Removal Kit (Applied Biological Materials, Canada) and cDNA synthesis was performed using 1 g of RNA per sample in a 20 l reaction using the 5X All-In-One RT MasterMix (Applied Biological Materials, Canada) according to the manufacturers instructions

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There are three well-known MAPK pathways in mammalian cells ( Figure 1 ): the ERK1/2, the c-JUN N-terminal kinase 1, 2 and 3 (JNK1/2/3), and the p38 MAPK , , , and pathways